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Here we show how the repeating pattern of DNA dyad symmetry, which occurs in the centromeric core of eukaryotic chromosomes, imposes a significant energetic penalty on long-range DNA looping. In particular, we use Monte Carlo simulations to calculate a strong dependence of both the equilibrium value of the looping energy and the looping loop size on the distances between the symmetry elements. In addition, we show that the presence of chromatin, which enhances looping, often results in entropically dominant looping modes, in which the length of the loop does not vary strongly with its overall size. However, we find that looping in the nucleosome is more susceptible to fluctuations than the looping in the chromatin fiber, thus explaining the conservation of the ∼10-100 bp-long loops found in the centromeric chromatin of mouse and human.Cajun grunts and roars
“Country food,” as defined by the U.S.D.A., “is a distinctive regional style or cooking that originated in the United States and that reflects the cooking traditions of a particular country or regions within the United States.” (“Country food,” in the U.S.D.A.’s Dictionary of Food, Alcohol, and Health, 1st ed., 2007, vol. 1, pg. 9.) I’ve had great luck with the hand-rolled cracklins, fried catfish, and sweet potato pie.
Nick Clooney, the chef and owner of Cajun Country Kitchen in Chardon, Ohio, has just published a new book: Americana: A Celebration of Country, American and Indigenous Food in the New Southern Kitchen (Andrews McMeel), which sits squarely in Cajun Country Kitchen’s kitchen, and is quite an occasion to pass the word about Cajun Country Kitchen.
The book’s cover and overall format are rife with Cajun iconography (the two cheeky frisky chickens against a red background, the woodstove, the pot hanging on the wall) which takes on an even more personal meaning when you read the book.
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