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Deoxyribonuclease II (DNase II) is an acid hydrolase that degrades DNA into nucleotides and the dinucleotide nucleoside 5′-monophosphate, and is responsible for the degradation of cell-free DNA from necrotic and apoptotic cells. A competitive oligonucleotide probe complementary to a part of the rat DNase II gene was tested in situ by in situ hybridization (ISH) on cryostat sections of normal and inflamed rat kidney. DNase II mRNA was detected in the cells of the proximal tubules of the cortex and the loop of Henle in the normal kidney. The strongest labeling was observed in the proximal tubules in the inner stripe and the loop of Henle. The nuclei of cells in the distal tubules and collecting ducts were negative. The hybridization signal in the inflamed kidney was comparable in intensity to that in the normal kidney. The expression pattern of DNase II mRNA in the inflamed kidney was similar to that observed in the previous studies of DNase II mRNA by in situ hybridization and Northern hybridization. In situ hybridization with a probe to the rat DNase II gene is an effective technique for detecting mRNA levels of DNase II in the normal and inflamed kidney.

The comeback has officially begun for Johnny Manziel.

On Sunday, the Browns announced that the Heisman Trophy winner had been activated from the injured reserve list, less than two months after tearing the anterior cruciate ligament in his right knee.

“It’s good to be back with the team,” Manziel said before the Browns’ 35

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