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The bone morphogenetic proteins (BMPs) trigger the differentiation of early mesoderm progenitors into osteoblast-lineage cells in vitro. We investigated the role of Notch signalling in osteogenesis by treating chick embryos and mouse embryonic stem (ES) cells with gamma-secretase inhibitors. DAPT-treated embryos exhibited reduced bone formation and decreased BMP-2 and -4 expression. DAPT treatment of ES cells impaired the bone-forming activity of these cells and markedly suppressed the expression of the bone-specific gene, osterix. A time-lapse videomicroscopic analysis revealed that osteogenesis can be divided into two phases: a rapid phase occurring in the blastoderm and over the next 12 hours; and a slower, post-implantation phase. Although DAPT treatment reduced the initial rate of differentiation, it did not affect the length of the rapid phase. We found that Notch signalling promoted the differentiation and survival of early osteoblasts, and that the BMP receptors and osterix were highly expressed in these cells.Sustainable Fashion

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